Mouse anti‑Rat CD1D IgG2a Monoclonal RW-C188719-0.1

Antibody: CD1D Mouse anti-Rat Monoclonal (Biotin) (WTH1) Antibody, Application: IHC-Fr, WB, IP, Flo, ELISA, Reactivity: Rat, Format: Biotin, Unmodified, Descriptio: CD1D antibody RW-C188719 is a biotin-conjugated mouse monoclonal antibody to rat CD1D. Validated for ELISA, Flow, IHC, IP and WB., Targe: Rat CD1D, Synonym: CD1D | CD1D antigen, d polypeptide | R3 | R3G1 | CD1d antigen | CD1d molecule | Thymocyte antigen CD1D, Hos: Mouse, Reactivit: Rat (tested or 100% immunogen sequence identity), Clonalit: IgG2a Monoclonal, Clon: WTH1, Conjugation: Biotin, Purificatio: Protein A purified, Modification: Unmodified, Immunoge: Rat CD1d-transduced M12.4.1. C3 cells., Specificit: Recognizes rat CD1d, a glycoprotein expressed on cortical thymocytes, certain T-cell leukemias and various other antigen presenting cells. CD1d binds self and non-self glycolipids and presents them to T-cell receptors on natural killer cells. When activated, natural killer cells produce Th1 and Th2 cytokines. Clone WTH1 recognizes a different, non-overlapping epitope than clone WTH2 (, or ), and binds with high affinity to the mature CD1d heavy chain independently of beta-2-microglobulin. Clone WTH1 efficiently blocks rat and mouse invariant natural killer T cell (iNKT cell) response to a-GalCer (an antigen for iNKT cells) and has a slightly higher affinity to native CD1d than clone WTH2. Clone WTH1 cross reacts weakly with mouse tissue in IHC and western blotting, but reacts strongly with both rat and mouse antigens in flow cytometry and immunoprecipitation., Application: IHC - Frozen
Western blot
Immunoprecipitation
Flow Cytometry (1:1000)
ELISA, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested., Presentatio: PBS, 0.02% Sodium Azide, Storag: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About CD1: Uniprot: P15813 NCBI: NM_001766 NP_001757.1