Pig anti‑Human Complement C3c Polyclonal RW-C147812-10

Antibody: Complement C3c Pig anti-Human Polyclonal Antibody, Application: IHC, IF, ELISA, Reactivity: Human, Format: Unconjugated, Unmodified, Other formats: FITC TRITC, Descriptio: Complement C3c antibody RW-C147812 is an unconjugated pig polyclonal antibody to human Complement C3c. Validated for ELISA, IF and IHC., Targe: Human Complement C3c, Hos: Pig, Reactivit: Human (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Unconjugated. Also available conjugated with FITC, TRITC., Purificatio: Purified IgG, Modification: Unmodified, Immunoge: Human C3c, Specificit: Human Complement C3c. In immunoelectrophoresis against fresh human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of human serum or plasma. Cross-reactivity The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail., Application: IHC
Immunofluorescence
ELISA, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: This product is intended for use as unlabelled primary or secondary antibody for the detection of C3c at the cellular and subcellular level by immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody in non-isotopic methodology and solid phase immunochemistry. Determinations of individual complement components can be useful in defining the exact location of a defect. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum working dilution should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000., Presentatio: Lyophilized from PBS, pH 7.2, Reconstitutio: Use 1 ml of sterile Distilled water., Storag: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.